Journal: Antioxidants

Article Title: Impact of Roux-en-Y Gastric Bypass on Mitochondrial Biogenesis and Dynamics in Leukocytes of Obese Women

doi: 10.3390/antiox11071302

Figure Lengend Snippet: Proinflammatory markers in obese patients before and after RYGB. Serum levels of ( A ) TNFα ( n = 32), ( B ) IL6 ( n = 36) and ( C ) IL1β ( n = 35). Leukocyte protein expression of inflammatory mediators and representative Western blot images of ( D ) MCP1 ( n = 13) and ( E ) NF-kB ( n = 21). Data are represented as the mean + SE. * p < 0.05, *** p < 0.001 when compared using a paired Student’s t -test. IL1β, interleukin 1β; IL6, interleukin 6; MCP1, monocyte chemoattractant protein 1, NF-κB, nuclear factor kB; RYGB: Roux-en-Y gastric bypass; TNFα, tumor necrosis factor alpha.

Article Snippet: Proteins of interest were detected by incubating membranes overnight at 4 °C with the following primary antibodies: mouse monoclonal anti-OPA-1 (Ref. MABN737), rabbit polyclonal anti-MFN1 (Ref.ABC41), rabbit polyclonal anti-MFN2 (Ref. ABC42) and rabbit polyclonal anti-FIS-1 (Ref. ABC67) from Merck-Millipore (Burlington, MA, USA); mouse monoclonal anti-OXPHOS (Ref. ab110411), rabbit monoclonal anti-MIEAP (Ref. ab180154), rabbit polyclonal anti-PGC1ɑ (Ref. ab54481) and rabbit polyclonal anti-MCP-1 (Ref. ab73866) from Abcam (Cambridge, UK); rabbit monoclonal phospho-DRP1 (Ser 616 ) from (Ref. 4494s) Cell Signalling Technology (Danvers, MA, USA); mouse monoclonal anti-mtTFA (Ref. sc-376672) from Santa Cruz (Dalas, TX, USA); mouse monoclonal anti-NF-кB (Ref. 33-9900) and rabbit polyclonal anti-GPX1 (Ref. PA5-30593) from Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Expressing, Western Blot